3D printed templates accommodate multiple thicknesses, from 50 μm up to several mm within the same template, with no additional processing cost or effort. Printed templates replace clean room and photolithographic fabrication resources and deliver resolutions of 50 μm, and up to 10 μm in localized hindrances, whereas the templates are smooth enough to allow direct transfer and proper sealing to glass substrates. The fabrication of conventional PDMS on glass lab-on-a-chip (LOC) devices, using templates printed with a commercial (2299 US$) micro-stereo lithography 3D printer, is demonstrated.